ABSTRACT
Although it has been known that seminal plasma (SP) modulates immune responses, the morphologic and functional effects of SP on mast cells have not been well documented. The purpose of this study was to determine the morphologic and functional effects of SP from the azoospermic (ASP), oligozoospermic (OSP) and normozoospermic (NSP) men on rat peritoneal mast cells (RPMC). By inverted microscopy, degranulation of RPMC occurred by treatment of OSP, NSP or compound 48/80. Treatment of RPMC with OSP and NSP resulted in increase in size and decrease in surface folds and bulged granules from the cell surface indicating that degranulating processes took place. Irregular cell surface with few microvilli and large degranulation channels filled with altered granules of various sizes were observed in OSP or NSP-treated RMPC. The degranulation index (DI) of RPMC treated with HBSS was 8.8+/-5.0. SP-induced DI was 11.9+/-6.2, 57.1+/-16.9 and 61.9+/-15.8, in ASP, OSP and NSP, respectively. The DI of RPMC treated with compound 48/80 (1 microgram/ml) was 83.5+/-21.4. These results indicated that the OSP, NSP but not ASP, induced the degranulation of RPMC. OSP and NSP induced histamine release (HR) from RPMC (4.5x105 cells). The HR of RPMC treated with HBSS was 1,030+/-196.6ng/ml, whereas ASP, OSP and NSP-induced HR was 1,010+/-204.7, 2,794+/-453.3 and 2,899 +/-366.7 ng/ml, respectively. The HR of RPMC treated with compound 48/80 was 6,300+/-476.2 ng/ml. Intracellular calcium level(ICL) of RPMC was also increased by OSP and NSP. The ICL of RPMC treated with HBSS was 6.1+/-1.0 pmole. ASP, OSP and NSP-induced ICL was 9.0+/-1.1, 81.2+/-18.5 and 76.6+/-18.0 pmole, respectively. The ICL of RPMC treated with compound 48/80 was 102.9+/-22.2 pmole. From the above results, it is suggested that OSP and NSP contain some factors to degranulate RPMC by the increase of intracellular calcium level.
Subject(s)
Animals , Humans , Male , Rats , Calcium , Histamine Release , Histamine , Mast Cells , Microscopy , Microvilli , Semen , ViperidaeABSTRACT
Simple posterior hip dislocations requiring open reduction is very few and reported approximately 3% of all posterior hip dislocations and we have little comments of the various causes preventing or blocking a concentric reduction following traumatic posterior hip dislocation. In a review of eleven cases of posterior dislocation requiring open reduction, we divided two groups, one is a failed closed reduction group, the other is a nonconcentric reduction group. A concentric reduction was prevented from an inverted limbus or an osteocartilagenous loose body from acetabulum or femoral head.
Subject(s)
Acetabulum , Joint Dislocations , Head , Hip Dislocation , HipABSTRACT
The femoral neck fracture in children is rare and occurred by severe trauma and the treatment method and prognosis are different from adult, and it is difficult to treat due to frequent complication, which is so called “unsolved fracture”. Twelve cases of children's femoral neck fracture were treated at Chon Buk National University Hospital from July, 1981 to May, 1988 were analysed clinically and radiologically and following results were obtained 1. The commonest age group was between the age of 14 and 16 years and the ratio of boys and girls was 2:l. 2. The main cause of fracture was traffic accident(6 cases) and fall down was 5 cases and slip down was 1 case. 3. According to the Delbet and Colona's classification, the transcervical fracture was most common type and displaced fracture was 8 cases. 4. Associated injuries were extremity fracture(3 cases) and pelvic bone fracture(2 cases) and head injury(2 cases), etc. 5. Seven cases were treated by open reduction and internal fixation and five cases by cast immobilization after skin or skeletal traction. 6. The commonest complication was coxa vara(5 cases) and other complication was avascular necrosis of femoral head(3 cases) premature epiphyseal closure(3 cases), nonunion (2 cases). 7. According to Ratliff's assessment of results, eight cases were good result and two fair results and two poor results. 8. Secondary operation (muscle pedicle bone graft, corrective subtrochanteric valgus osteotomy) was done in three cases and showed one good result, one fair result and one poor result.